Briefly describe the system you would like to study and the specific questions you would like to answer:
List the NMR active nuclei that you are interested in studying (1H, 13C, 15N or 31P). List any specific NMR experiments that you are interested in conducting. If you cannot answer either of these questions, answer "unsure".
Include details about the source of the macromolecules you plan to study (amounts of purified protein that can be obtained, extent of purity and the means by which this estimate was obtained, if prepared by recombinant means, host system and yield (in milligrams) per liter of culture medium).
Describe any characterization of the macromolecules you have conducted (bioassays or biophysical characterization, such as molecular weight by mass spectrometry or behavior in solution by analytical ultracentrifugation)
Include details about the molecular weights, solubility, and sample conditions under which you would like to conduct your studies (click here for guidelines for sample preparation).
Are you aware of any NMR studies that have already been published on this system or highly related systems? If so include literature references and explain how your work is different or complementary to that already published
Do you have any NMR experience? If so, would you desire training or assistance from personnel associated with the NMR facility. If not, are you interested in being trained to use the NMR facility?
Is your laboratory already funded to support this research, or will the data collected in the NMR facility be usedto support a grant application?
Any additional comments that may be useful in evaluating the feasibility of the conducting NMR studies on this system.
Center for Biomolecular Structure Analysis
Department of Biochemistry
Last updated on
August 25, 2005